Table 5.

Single nucleotide polymorphisms (SNPs) from F. psychrophilum CSF 259–93 strain passaged with no rifampicin (CN)

Reference positiona	Allele frequency (in %)b	SNPc	Coveraged	Annotatione	Amino acid changee	Gene/Proteinf
575838	100	C > G	71	FPSM_00502	Val638Leu	Ribonucleoside-diphosphate reductase large chain (nrdA)
824811	94	A > G	50	FPSM_00723	Ile62Thr	GTP-binding protein YihA
2606362	100	G > T	25	FPSM_02361	Trp4Leu	Putative membrane spanning protein
2606399	95	A > T	20	FPSM_02361	Leu16Phe
2718910	100	T > C	83	FPSM_02464	Tyr418Cys	Di-tripeptide transporter

^aReference position (in bp) for reference sequence (listed under annotation field) are based on CSF 259–93 sequence from ERGO-Integrated genomics

^bAllele frequency refers to the proportion of sequences showing a given single-nucleotide polymorphism (SNP) at the started reference position

^cShows the SNP change represents the change from nucleotide X to Y (X > Y) at the reference position

^dCoverage refers to the total number of sequencing reads that align to each base within the sample DNA

^ePredicted amino acid change for the identified SNP

^fAnnotation shows the name and putative function for the identified gene. Annotation is presented from the CSF 259–93 sequence from ERGO-Integrated genomics