. 2015 Apr 22;1(2):89–93. doi: 10.1021/acscentsci.5b00056

Table 1. Total Turnovers (TTN) to Product for Aziridination Catalyzed by Purified Holoenzymes P411_BM3-CIS-T438S (P) and P411_BM3-CIS-T438S-I263F (P-I263F) with Selected Styrenyl Olefins 1, 3, and 5 and Tosyl Azide^a.

	TTN^b
enzyme	2	4	6
P411_BM3-CIS-T438S (P)	15	8	5
P-I263F	150	160	190

Reactions were performed in 0.1 M KPi buffer pH = 8.0 using 0.2 mol % enzyme and NADPH as reductant, with 2.5 mM tosyl azide and 7.5 mM olefin. Detailed reaction conditions can be found in the Supporting Information.

TTN = total turnover number. TTNs were determined by HPLC analysis.

Table 1. Total Turnovers (TTN) to Product for Aziridination Catalyzed by Purified Holoenzymes P411BM3-CIS-T438S (P) and P411BM3-CIS-T438S-I263F (P-I263F) with Selected Styrenyl Olefins 1, 3, and 5 and Tosyl Azidea.

Table 1. Total Turnovers (TTN) to Product for Aziridination Catalyzed by Purified Holoenzymes P411_BM3-CIS-T438S (P) and P411_BM3-CIS-T438S-I263F (P-I263F) with Selected Styrenyl Olefins 1, 3, and 5 and Tosyl Azide^a.