Table 2. Improvement in Yield and % ee for Aziridine Product 4 with Active-Site Evolution of P411_BM3-CIS-T438S (P)^a.

		% yield
entry	enzyme	4	7	% ee of 4b
1	no enzymec	0	95	nd
2	P411BM3-CIS-T438S	1.1	95	25
3	P-I263F	40	54	55
4	P-I263F-A328V	43	50	96
5	P-I263F-L437V	37	52	95
6	P-I263F-A328V-L437V	55	43	99

^aReactions were carried out using whole E. coli cells resuspended in M9-N reaction buffer under anaerobic conditions, with 2.5 mM tosyl azide and 7.5 mM 4-methylstyrene. Yield is based on tosyl azide. See Supporting Information methods for detailed reaction setup and quantification procedures.

^b% ee determined by SFC analysis and calculated as (S – R)/(S + R).

^c“No enzyme” reactions were carried out using whole cells with no P411 enzyme expressed, as described in the Supporting Information methods.