Table 3.
Central composite design for optimization of independent variables affecting curd formulation with the observed dependent responses
| Run | Independent variables | Responses | |||||
|---|---|---|---|---|---|---|---|
| A | B | Y1 | Y2 | Y3 | Y4 | Y5 | |
| 1 | 12 | 3 | 2.21 | 6.28 | 6400 | 94.9 | 0.54 |
| 2 | 12 | 7 | 2.384 | 6.16 | 6400 | 95.2 | 0.72 |
| 3 | 24 | 3 | 3.666 | 4.80 | 102400 | 97.1 | 0.81 |
| 4 | 24 | 7 | 3.733 | 4.66 | 102400 | 97.8 | 0.99 |
| 5 | 9.51472 | 5 | 2.42 | 6.31 | 6400 | 92.0 | 0.45 |
| 6 | 26.48528 | 5 | 3.514 | 4.45 | 204800 | 97.8 | 0.90 |
| 7 | 18 | 2.1715729 | 3.135 | 5.26 | 12800 | 98.0 | 0.63 |
| 8 | 18 | 7.8284271 | 2.844 | 4.92 | 25600 | 99.0 | 0.90 |
| 9 | 18 | 5 | 2.445 | 4.32 | 12800 | 95.6 | 0.81 |
| 10 | 18 | 5 | 2.413 | 4.38 | 12800 | 95.0 | 0.81 |
| 11 | 18 | 5 | 2.4 | 4.35 | 12800 | 95.4 | 0.81 |
| 12 | 18 | 5 | 2.435 | 4.34 | 12800 | 95.0 | 0.81 |
A incubation time (h), B inoculum level (%v/v); Y 1 cell growth (OD600), Y 2 pH, Y 3 bacteriocin activity (AU/ml), Y 4 DPPH free radical scavenging activity and Y 5 Total titrable acidity (gm/ml)