Table 3.
Total phenolic, mimosine and chlorophyll contents of water extracts from brown lead seed as affected by drying methods
| Yield / compositions | Oven drying | Freeze drying | ||
|---|---|---|---|---|
| Without PCRb | With PCR | Without PCR | With PCR | |
| Extraction yield (%) | 8.7 ± 0.00abB* | 10.0 ± 0.00aA | 8.4 ± 0.00bB | 10.0 ± .0.00aA |
| Total phenolic content (g GA equivalent /100 g samplec) | 45.2 ± 0.03dB (3.9 ± 0.00dB)** | 57.4 ± 0.02cA (5.7 ± 0.00bA) | 62.6 ± 0.00bB (5.3 ± 0.00cB) | 72.9 ± 0.00aA (7.3 ± 0.00aA) |
| Mimosine content (mmol mimosine / 100 g samplec) (spectrophotometric method) | 26.9 ± 0.25cA (2.3 ± 0.02dB) | 25.6 ± 0.21cA (2.6 ± 0.02cA) | 258.2 ± 0.00aA (21.7 ± 0.00bB) | 221.5 ± 0.00bB (22.2 ± 0.00aA) |
| Mimosine content (mmol mimosine / 100 g samplec) (HPLC method) | 25.4 ± 0.07cA (2.2 ± 0.01dB) | 24.1 ± 0.02cA (2.4 ± 0.00cA) | 251.3 ± 0.09aA (21.1 ± 0.01bB) | 216.7 ± 0.04bB (21.7 ± 0.00aA) |
| Chlorophyll content (mg/100 g extract) | 11.5 ± 0.00bA (1.0 ± 0.00bA) | 3.2 ± 0.03dB (0.3 ± 0.00dB) | 23.8 ± 0.03aA (2.0 ± 0.00aA) | 9.2 ± 0.03cB (0.9 ± 0.00cB) |
*Different lowercase and uppercase letters within the same row and the same drying method, respectively, indicate significant differences (P < 0.05)
**Different lowercase and uppercase letters in the parenthesis within the same row and the same drying method, respectively, indicate significant differences (P < 0.05)
aValue are mean ± standard deviation (n = 3)
bPrior chlorophyll removal
cValues without and in the parenthesis were calculated with reference to the extract and brown lead seed (dry weight), respectively