Table 2.
Restriction enzymes with their detailed description of the fragments that could discriminate the most commonly used meat animal species
| Species | Restriction enzyme that digests the 609 bp PCR product (size in bp) | |||||
|---|---|---|---|---|---|---|
| Alu I | Taq I | |||||
| RE site (s) No. & position | Fragments (bp) | RE site (s) No. & position | Fragments (bp) | |||
| Expected | Diagnostic | Expected | Diagnostic | |||
| Cattle | (2) 92 | (3) 64 | (1) 453 | (1) 315 | (2) 294 | (2) 294 |
| 545 | 92 | 315 | 315 | |||
| 453 | ||||||
| Buffalo | (1) 92 | (2) 92 | (1) 517 | (1) 70 | (2) 70 | (1) 539 |
| 517 | 539 | |||||
| Goat | – | – | – | (3) 43 | (4) 43 | (3) 131 |
| 315 | 131 | 163 | ||||
| 446 | 163 | 272 | ||||
| 272 | ||||||
| Sheep | (1) 350 | (2) 259 | (2) 259 | (1) 315 | (2) 294 | (2) 294 |
| 350 | 350 | 315 | 315 | |||
| Pig | (3) 17 | (4) 17 | (2) 243 | (2) 43 | (3) 43 | (2) 267 |
| 260 | 27 | 322 | 342 | 267 | 299 | |
| 287 | 243 | 299 | ||||
| 322 | ||||||