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. Author manuscript; available in PMC: 2015 Sep 16.
Published in final edited form as: Mol Microbiol. 2011 Nov 21;82(6):1481–1495. doi: 10.1111/j.1365-2958.2011.07902.x

Figure 4. Vfr represses SpeB production by a product present in the culture supernatant.

Figure 4

(A) speB transcript level analysis of mutant strain Δvfr grown in the culture supernatant the indicated strains for 1 hr as described in Materials and Methods. Two biological replicates were performed on two separate days and analyzed in duplicate with data graphed being mean ± standard deviation. P value is derived from a two-sample t-test. (B) Western immunoblot analysis of SpeB in the growth medium of mutant strain Δvfr grown for 30 minutes and 60 minutes in the culture supernatant derived from indicated strains as described in (A). (C) Western immunoblot analysis of SpeB in the growth medium of mutant strain Δvfr grown for 60 minutes in either the total culture supernatant or culture supernatant filtered with YM-3 (< 3 kDa) membrane derived from indicated strains. Proteinase K treated culture supernatants (Prot K) used for swapping are indicated.