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. Author manuscript; available in PMC: 2015 Sep 16.
Published in final edited form as: Mol Microbiol. 2011 Nov 21;82(6):1481–1495. doi: 10.1111/j.1365-2958.2011.07902.x

Table 1.

Bacterial strains and plasmids used in this study.

Strain or plasmid Description Reference
Strains
    MGAS10870 Invasive isolate, serotype M3 (Beres et al., 2010)
    MGAS10870:pDC MGAS10870 with empty vector, Cm+ This study
                Δ vfr MGAS10870Δvfr::aad9 This study
    Δvfr:pDC MGAS10870Δvfr::aad9, empty vector, Cm+ This study
    Δvfr-pDC:vfr MGAS10870Δvfr::aad9, vfr+, Cm+ This study
    Δvfr-pDC:pep MGAS10870Δvfr::aad9, Vfr peptide+, Cm+ This study
    MGAS10870ΔspeB MGAS 10870ΔspeB::aad9 This study
Plasmids
    pSL60-1 Vector containing aad9 gene encoding spectinomycin resistance (Lukomski et al., 2000)
    pDC123 Low-copy number plasmid capable of replication in GAS and Escherichia coli, Cm+ (Li et al., 1997)
    pDC:vjr pDC123 with entire vfr gene plus promoter, Cm+ This study
    pDC:pep pDC123 with Vfr N-terminal secretion signal plus promoter, Cm+ This study