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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: J Immunol. 2015 Sep 9;195(8):3531–3536. doi: 10.4049/jimmunol.1500626

FIGURE 1. Antigen recognition reduces the tissue egress of CD4+ effector T cells.

FIGURE 1

(A) Different ratios of OVA-specific OTII Th1 cells and OVA-pulsed APCs (constant per recipient) were co-incubated and subcutaneously injected into the footpads of WT recipient mice. Footpad thickness was measured and corrected for pre-injection values (Δ swelling). Data points show mean ± SEM (5 mice per group) in one representative of 3 performed experiments. (B–C) A mixture of CFSE-labeled polyclonal CD45.1+ and OTII CD45.2+ Th1 cells were co-incubated with BSA- or OVA-pulsed APCs before injection into the footpads of recipient mice. 20 h later, donor cells that had egressed from the effector site and reached the draining lymph node (dLN) were enumerated by flow cytometry. Migration of OTII Th1 cells relative to polyclonal Th1 cells (B) in one representative experiment (5 mice per group) or (C) in all (n=4) independent experiments combined and expressed relative to the mean ratio of migrated OTII Th1 cells to polyclonal Th1 cells in the presence of BSA (set as 100%). Data points indicate individual recipients and the mean ± SEM of each group. ****P < 0.0001.