FIGURE 1:

The identification of mutants affecting the spatial organization of the endocytic and/or exocytic pathways. (A) Steady-state images of fixed cells at different cell cycle stages. Scale bars, 5 μm. Bar charts show the percentage of polarized cells normalized to control levels. (B) Mutants predominantly affecting endocytosis, exocytosis, or both pathways. Percentage of cells with polarized endocytic and exocytosis (normalized to the control level) is shown as red and blue dots, respectively, with error bars showing the uncertainty in the fraction of (de)polarized cells (see Materials and Methods). The vertical dotted lines indicate the polarization level of control cells (gray) and the threshold (70% of control level) for endocytosis (red) and exocytosis (blue). ^†Strains shifted to 37ºC instead of 34°C before fixation.