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. 2015 Jul 1;26(13):2519–2534. doi: 10.1091/mbc.E14-11-1527

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© 2015 Jose et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Vesicle tracking in exocyst mutants reveals exocytic dynamics similar to that of endocytic mutants. (A) Top, vesicle trajectories in small-budded control and sec6-4 cells expressing mEos-Sec4. Lower (D = 0.01–0.03 μm²/s) and higher (D > 0.1 μm²/s) mobile vesicle trajectories are shown in cyan and magenta, respectively. Bottom, heat maps of D of exocytic vesicles in control and sec6-4 cells. Scale bar, log D values on the heat map. (B) Normalized histograms of log D of exocytic vesicles in control cells (black) and sec6-4 (blue) mutants. (C) Cumulative probability distributions of log D of exocytic vesicles in control cells (black) and sec6-4 (blue) mutants. (D) Average MSD curves for control cells (black) and sec6-4 mutants (blue). (E) Percentage of cells displaying interspersed endocytic and exocytic trafficking domains in different exocyst mutants. More than 100 small-budded cells were used for the control and each mutant in the statistics. The error bars indicate SE in B–D, and N is the number of trajectories.