FIGURE 3:

Xbp1-like SLs are not sufficient to target endogenous mRNAs to RIDD. MC3T3-E1 cells were treated with 1 mM DTT, 2 μg/ml actinomycin D (Act), or both for 4 h. We then measured relative mRNA levels of noted transcripts by qPCR. Transcripts were chosen based on their predicted localization to the ER (based on Gene Ontology term analysis) and the presence of Xbp1-like SLs, defined as 1) a seven-membered loop with the four conserved residues (as in Figure 2A), and 2) a stem of at least 5 base pairs including three GC pairs. The verified RIDD target Blos1 was also measured as a control. Shown are averages and SDs from two independent experiments. ECM, extracellular matrix; PM, plasma membrane; Ut, untreated.