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. 2015 Aug 15;26(16):2939–2954. doi: 10.1091/mbc.E15-04-0247

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© 2015 Barbosa, Carreira, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — CREB‑H is stabilized by dominant-negative Cul1. (a) CREB‑HΔTMC wt was expressed alone (lane 1) or coexpressed with Cul1 DN (Flag-tagged dominant-negative Cul1; lane 2), Fbw1a (Flag-tagged Fbw1a; lane 3), or a combination of the two (lane 4). Lysates were harvested and analyzed with anti-SV5 antibody to detect CREB‑H (top) or anti-Flag antibody to detect Cul1 DN and Fbw1a (bottom). (b) As for a, but now comparing the effects of Cul1 DN and Fbw1a on wt (lanes 1–3) vs. the DSG mutant (lanes 4–6).