Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 1;26(15):2742–2754. doi: 10.1091/mbc.E15-02-0094

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Perpelescu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 3: — Middle region of HJURP is essential for cell viability. (A) Scheme of different HJURP GFP-tagged expression constructs. The predicted position of α-helixes obtained with PSIPRED version 3.3 software. (B) Summary of results for localization and complementation assay of indicated GFP-tagged HJURP mutant proteins. Supplemental Figure S2 shows the results of cell viability assay. (C) Representative images showing cellular localization of different GFP-HJURP mutant proteins (green). CENP-T was used as a centromere marker (red). DAPI was used for DNA staining (blue). HJURP^ON refers to cells grown in the absence of tetracycline and HJURP^OFF to those grown for 48 h in the presence of tetracycline. Because cells expressing C-terminal truncation of HJURPs (HJURP^1-254 and HJURP^1-400) restrictively localize to the cytoplasm, it is possible that the middle region may be related to the nuclear localization of HJURP. (D) Western blot analysis with anti-GFP antibody showing the expression levels for each of the GFP-mutant proteins. Although GFP-tagged HJURP^1-500 shows unexpectedly slower migration, similar to GFP-tagged HJURP^FL, expression of GFP-tagged HJURP^1-500 was confirmed by genomic PCR.