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. 2015 Aug 1;26(15):2755–2768. doi: 10.1091/mbc.E14-06-1105

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© 2015 Nimlamool et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — MAPK and PKC mediate VEGF-induced Cx43 phosphorylation. (A) PAECs were left untreated, treated with VEGF for 15 min, or incubated with VEGF receptor inhibitor PD98059 or GF109203X 1 h before VEGF treatment prior to cell lysis and Western blot analyses. Phosphorylation of Cx43 was detected using phosphospecific antibodies directed against Ser-255, Ser-262, Ser-279/282, and Ser-368. Phosphorylation of ERK1/2 was also detected using anti–phospho ERK1/2–specific antibodies. Representative Western blots. (B) Normalized quantitative analyses of phosphorylated Ser-255, Ser-262, Ser-279/282, and Ser-368 of Cx43 of three independent experiments. Note that VEGF-mediated Cx43 phosphorylation is reduced significantly when VEGF receptors or MAPK or PKC signaling pathways were inhibited.