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. 2015 Aug 1;26(15):2788–2800. doi: 10.1091/mbc.E15-01-0018

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© 2015 Awata, Song, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — DRC3 is in a subcomplex with DRC4, DRC7, and DRC11. Isolated axonemes of WT and the DRC3-HA strain were extracted with KI and the extract subjected to immunoprecipitation using the anti-HA antibody. (A) Silver-stained SDS–polyacrylamide gel of the resulting immunoprecipitate. An arrow indicates a band likely to contain HA-tagged DRC3. Arrowheads indicate bands with polypeptides specifically coprecipitated with HA-tagged DRC3. (B) The same samples as in A were analyzed by Western blotting using anti-DRC3, 4, 7, and 11 antibodies.