FIGURE 8:
DRC3 is in a subcomplex with DRC4, DRC7, and DRC11. Isolated axonemes of WT and the DRC3-HA strain were extracted with KI and the extract subjected to immunoprecipitation using the anti-HA antibody. (A) Silver-stained SDS–polyacrylamide gel of the resulting immunoprecipitate. An arrow indicates a band likely to contain HA-tagged DRC3. Arrowheads indicate bands with polypeptides specifically coprecipitated with HA-tagged DRC3. (B) The same samples as in A were analyzed by Western blotting using anti-DRC3, 4, 7, and 11 antibodies.