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. 2015 Aug 1;26(15):2823–2832. doi: 10.1091/mbc.E15-01-0009

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© 2015 Maguire et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — cil-7 mutants accumulate EVs in the cephalic lumen but are not defective in ciliogenesis. (A–D) Series of TEM images in a wild-type animal displaying EVs in the sheath cell lumen that surrounds the CEM and CEP distal dendritic region (lumen is pseudocolored yellow). (A′–D′) TEM images revealed that cil-7(tm5848) males had excessive amounts of EVs in the sheath cell lumen. Both CEM and CEP axonemes appeared normal. (E) Cartoon depiction of the cil-7(tm5848) mutant phenotype compared with wild type, including increased quantity of EVs and distended sheath cell lumen. Dashed lines indicate the location of the A–D and A′–D′ TEM images with respect to the cephalic sensillum. (F) Compared to wild type (0.6 ± 0.3 μm³), the cil-7(tm5848) sheath cell lumen is 10 times larger (5.8 ± 1.5 μm³ [average volume ± SD]; n = number of cephalic sensilla). (G) The average CEM volume enclosed within the sheath cell that surrounds the CEM distal dendrite and axoneme in wild type was 0.2 ± 0.1 μm³, and the corresponding volume in cil-7(tm5848) was 0.24 ± 0.2 μm³ (average volume ± SD; n = number of cephalic sensilla), indicating that the integrity of CEM neurons is normal in cil-7 mutants. (H) Compared to wild type, cil-7(tm5848) animals displayed an increased number of EVs in the sheath cell lumen. The frequency distribution of EV sizes in cil-7(tm5848) was slightly favored toward EVs above the average mean (skewness of the diameter distribution as measured in Excel: wild type, 0.46; cil-7, 0.57). (I) The average EV diameter of wild-type and cil-7(tm5848) males was not significantly different. The average EV diameter for wild type was 129.1 ± 49.4 nm and the average vesicle diameter for cil-7(tm5848) was 131.8 ± 43.3 nm (average diameter ± SD). In F, ***p < 0.001 by the unpaired t test. In G, a nonsignificant difference was determined by the unpaired t test. In I, a nonsignificant difference was determined by Welch's t Test. Box-and-whisker plots are included in I, where the plot is divided into limits that are each 25% of the population.

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