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. 2015 Aug 1;26(15):2833–2844. doi: 10.1091/mbc.E15-01-0053

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© 2015 Casey et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — lnp1Δ rtn1Δ cells have defects in NPC organization. (A) Parental or mutant cells expressing Nic96-GFP were grown to early log phase at 25°C and visualized by fluorescence microscopy. Scale bar, 5 μm. (B) The aggregation indexes of Nic96-GFP–expressing cells. Error bars represent SE. Asterisk denotes statistical significance (p < 0.01). n.s., no statistical significance. (C) Percentages of cells with regions of collapsed cortical ER as indicated by lack of peripheral Sec61-GFP staining as quantified from images of Sec61-GFP–expressing cells. Error bars represent SE. Asterisk denotes statistical significance (p < 0.01). (D) Parental or mutant cells expressing Sec61-GFP grown to early log phase at 25°C and visualized by fluorescence microscopy. Scale bar, 5 μm. Arrows mark typical regions of collapsed cortical ER in represented cells. Arrowheads for lnp1Δ rtn1Δ highlight aberrant folds in cortical ER.