Proliferation of PEC, BPH-1, and LNCaP cells is increased on aged collagen relative to young collagen. PEC and BPH-1 cells cultured for 72 h on young (A, B) or aged (D, E) collagen gels assumed a flattened morphology. In contrast, LNCaP cells cultured for 72 h on young (C) or aged (F) collagen gels had rounded contours. Proliferation measured at 72 h shows that all three cell lines: PEC (G), BPH-1 (H), and LNCaP (I) proliferated at a significantly greater rate on aged collagen (AC—gray bars) than on young collagen (YC—black bars). Values are mean± standard deviation (*p<0.05, **p<0.005). A–F scale bars=50 µm.