Table 2.

Comparison of behavioral and physiological changes between the Shank3^ΔC exon 21 deletion mouse model (Kouser et al., 2013) and our present Shank3^G exon 21 insertion mutation model

Behavior	Behavioral parameter	Shank3ΔC (Kouser et al., 2013) (WT/Homo)	Shank3G (WT/Homo)	Shank3G (WT/Het)
Body weight	Body weight in grams	↓	↓	–
Open field	Time in center/time in periphery	–	–	–
	Distance traveled	↓	↓	–
	Velocity	↓	↓	–
Dark/light	Time spent in dark chamber	↑	–	–
	Time spent in light chamber	↓	–	–
	Latency to enter light chamber	↑	↑	–
	Distance traveled	↓	↓	–
Elevated plus maze	Time in open arms/both arms	–	–	–
	Distance traveled	–	↓	–
	Velocity	–	↓	–
Morris water maze	Training: latency to platform	↑	↑	–
	Training: distance traveled	↑	↑	–
	Training: thigmotaxis	↑	–	–
	Training: velocity	–	↑	↑
	Probe: % time in target quadrant	↓	–	–
	Probe: frequency of target platform location crossing	–	–	–
	Probe: thigmotaxis	↑	–	–
	Probe: velocity	–	–	–
Nesting	Nest width	↓	↓	–
	Nest height	↓	↓	–
Three choice social interaction test	Front versus back target	–	–	–
	Social versus inanimate target	–	–	–
	Familiar versus novel target	↓	–	–
Locomotor test	2 h	–	–	–
	First 5 min	↓	↓	–
Rotarod	Motor learning	–	↓	–
	Motor coordination	↓	↓	–
Marble burying	No. of buried marbles	↓	↓	–
Grooming	Time spent grooming	–	–	–
	No. of bouts	–	–	–
	Time per bout	–	–	–
Electrophysiology
	I/O curves	↓	↓	↓
	PPR	–	–	–
	LTP	↓	–	–
	mGluR-LTD	–	↓	–
	mEPSC amplitude	–	–	–
	mEPSC frequency	↓	↓	–
	NMDA/AMPA	↓	↓	–