Figure 3.

miR-155 deletion diminishes LPS-induced microglial activation and IL6 protein levels. A, Representative images of inflammation-induced changes in microglial morphology (ionized calcium binding adaptor protein-1, IBA1, green, microglial marker; DAPI, blue, nuclei marker) in the DG (including GCL, SGZ, and hilus) of WT and miR-155^−/− (155KO) mice treated with saline or LPS (1 μg) intracerebroventricular at 7 weeks of age. Top left, Labeling of GCL and hilus; refer to Figure 2A for the diagram of hippocampus. B, Quantification of total IBA1⁺ cells in DG (including SGZ, GCL, and hilus; Fig. 2A) normalized to the size of area. C, IBA1⁺ microglial cell morphology shown as proportion of amoeboid out of total IBA1⁺ cells. D, IL6 protein expression in whole brain measured by ELISA. n = 3 animals per group; ****p < 0.0001 and *p < 0.05 as determined by 1-way ANOVA and Tukey post hoc. Data are shown as mean ± SEM.