Table 3.

qRT-PCR validation of microarray data by using the data-generated RNA samples.

Gene symbol	Microarray ratio		qRT-PCR ratio
	O versus M	O versus Y	O versus M	t-test (P value)	O versus Y	t-test (P value)	RefSeq transcript ID
Upregulated genes
MRAP	—	22.8616	19.1597	0.030∗	45.2548	0.027∗	XM_001096328
MMP9	—	5.2734	14.4765	0.815	4.2191	0.002∗∗	XM_001104871
THY1	—	2.8757	1.3062	0.273	1.6950	0.098	NM_001042638
LOC717872	—	7.0041	1.3145	0.054	3.3168	0.073	XM_001109859
IGFBP4	—	2.2457	0.8946	0.417	1.4958	0.403	XM_001097914
Downregulated genes
NASP	0.4166	0.3654	0.2207	0.0001∗∗	0.2588	0.0001∗∗	XM_001103253
PTTG1	0.2017	0.1803	0.2505	0.045∗	0.2811	0.002∗∗	XM_001085022
AURKA	0.3774	0.2893	0.3221	0.043∗	0.1231	0.002∗∗	XR_011039
BCL2L10	0.0369	0.0279	0.0000	0.001∗∗	0.0000	0.032∗	XM_001085850
BARD1	0.3524	0.2966	0.5953	0.193	0.2293	0.015∗	XM_001084147
TACC3	0.1866	0.1238	0.1756	0.004∗∗	0.0884	0.004∗∗	XM_001101192
THBD	0.4251	0.4500	3.5801	0.160	3.8106	0.845	XM_001095416
DNMT3A	0.3844	0.3167	0.1805	0.0004∗∗	0.0634	0.0001∗∗	XM_001083234
ZP3	0.0804	0.0347	0.0005	0.020∗	0.0003	0.0002∗∗	XM_001114760
LOC703074	0.0294	0.0265	0.0010	0.001∗∗	0.0012	0.001∗∗	XR_010378
WASF1	0.2458	0.2101	0.3482	0.0001∗∗	0.1213	0.0001∗∗	XM_001087511
LOC707199	0.4462	0.3053	0.7749	0.435	0.2752	0.041∗	XR_011694
MCM3	0.2856	0.212	0.3024	0.0001∗∗	0.0734	0.002∗∗	XM_001106702
IGF2BP3	0.1042	0.0617	0.2369	0.092	0.0993	0.024∗	XM_001098017
FGF14	0.1707	0.1074	0.2601	0.005∗∗	0.0696	0.002∗∗	XM_001093457
XRCC6	—	0.4987	0.8063	0.855	0.5597	0.146	XM_001105684
HELLS	—	0.2874	0.227	0.0001∗∗	0.1330	0.0005∗∗	XM_001094077
CDK1	—	0.0942	0.1716	0.0001∗∗	0.1137	0.0005∗∗	XM_001095697
UBE2C	—	0.1019	0.3849	0.016∗	0.1806	0.001∗∗	XM_001104061
THBS1	0.3518	—	0.3748	0.001∗∗	0.1309	0.069	XM_001093770

O: old group; M: middle-aged group; Y: young group; —: no significant difference in gene expression; ^∗ P < 0.05; ^∗∗ P < 0.01.