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. 2015 Aug 31;5:733–740. doi: 10.1016/j.fob.2015.08.010

Fig. 3.

Fig. 3

Regulation of MeCP2 by miRNAs. (A) Taqman analysis of Mecp2 in Neuro-2a cells 48 h after miRNA transfection showed no significant reduction in mRNA (n = 3). (B) Western blot analysis after transfection of Neuro-2a cells with miR-301, miR-132 and miR-19a significantly reduced MeCP2 protein (n = 3). (C) MeCP2 expression increased when cells were transfected with anti-miR (miRNA inhibitors) for miR-301 and miR-132; (n = 3, representative blot shown). (D and E) Immunocytochemisty and quantification for MeCP2 in Neuro-2a cells transfected with miR-132. (F and G) Immunocytochemistry and quantification for MeCP2 in Neuro-2a cells transfected with miR-19a. (H and I) Immunocytochemistry and quantification for MeCP2 in Neuro-2a cells transfected with miR-301. MeCP2 levels were normalized to DAPI staining of the nucleus and the graph represents MeCP2 expression relative to untransfected cells. Representative images are shown, n = 3. Significance was determined using Student t-test, p value *<0.05, **<0.001 ***<0.001.