Fig. 5.

MeCP2 T158A knockin mice have decreased mechanical sensitivity and lower Bdnf. (A) Mechanical sensitivity measured by von Frey filaments showed an increase in withdrawal threshold in 12 weeks old MeCP2 T158A mice, indicating hyposensitivity compared to wild-type littermate controls (n = 5 for MeCP2 T158A mice and n = 7 for wild-type littermate). Statistically significant difference was determined using the Mann–Whitney U test; there was a significant reduction in withdrawal threshold in the ipsilateral paw p value *<0.02. (B) DRG from Mecp2-null mice and (C) MeCP2 T158A mice has significantly lower Bdnf mRNA compared to wild-type littermates, (n = 6). (D) Bdnf was also lower in MeCP2 T158A mice compared to wild-type littermates after SNI surgery. Significance determined using Student t-test, p value *<0.05 (n = 3 for both MeCP2 T158A mice and wild-type littermate).