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. 2015 Sep 16;5:14075. doi: 10.1038/srep14075

Figure 2.

Figure 2

(a) Representative fluorescence transients of eYFP in four different buffer conditions: t50 (Tris, pH 7.4), 70 mM ME, enzymatic depletion of oxygen, and dSTORM-buffer (depletion of oxygen and 70 mM ME). (b) Mean number of switching cycles of single eYFP prior to bleaching. (c) Mean number of photons of eYFP per ON-time. (d) Mean lifetimes of ON- and OFF-states, tON, tOFF of single eYFPs. (b–d) Mean values were obtained from exponential fitting of histograms of more than 100 individual proteins colocalized with one ATTO647N dye. The error of the fits is indicated.