Figure 3.

Losses in motor function in parkin Q311X mutants are abrogated by rapamycin feeding. Motor coordination and balance were assessed in parkin mutant mice (Mut) versus WT littermate controls on control or rapamycin diet (Rapa) via the pole test as previously described by our laboratory (Lieu et al., 2013). Time to turn downward on the pole (A) and to descend to the base (B) was measured over 5 consecutive trials. ***p < 0.001 versus WT. ⁺⁺⁺p < 0.001 versus Mut. C, Spontaneous activity was measured in Mut versus WT littermates on control or Rapa diet as previously described in these animals by Lu et al. (2009) using a Tru Scan photobeam apparatus (Colbourn Instruments) in five separate trials. **p < 0.01 versus WT. ⁺⁺⁺p < 0.001 versus Mut.