Table 3.
Gene expression comparison between Prox1 heterozygous versus null in CGE-derived interneuron precursors within the E18.5 cortex
| Symbol: Name | Fold change |
|---|---|
| Genes upregulated in CGE-derived Prox1 loss-of-function cells in the E18.5 cortex | |
| XIST: X inactive-specific transcript (nonprotein coding) | 376.517 |
| SLN: sarcolipin | 5.321 |
| SFRP2: secreted frizzled-related protein 2 | 4.251 |
| SCN1A: sodium channel, voltage-gated, type I, α-subunit | 3.998 |
| C1QTNF1: C1q and tumor necrosis factor-related protein 1 | 3.558 |
| NTNG1: netrin G1 | 3.009 |
| PROX1: prospero homeobox 1 | 2.717 |
| DLK1: δ-like 1 homolog (Drosophila) | 2.709 |
| MSRB2: methionine sulfoxide reductase B2 | 2.384 |
| SVIL: supervillin | 2.377 |
| DUSP26: dual specificity phosphatase 26 (putative) | 2.287 |
| TGFBI: transforming growth factor, β-induced, 68 kDa | 2.15 |
| Etl4: enhancer trap locus 4/KIAA1217 | 2.142 |
| GABRA2: GABA A receptor, α2 | 2.14 |
| DBC1: deleted in bladder cancer 1 | 2.083 |
| FGA: fibrinogen α chain | 2.034 |
| Rnd3: Rho family GTPase 3 | 1.991 |
| SEZ6L: seizure-related 6 homolog (mouse)-like | 1.958 |
| HCN1: hyperpolarization activated cyclic nucleotide-gated potassium channel 1 | 1.926 |
| PAG1: phosphoprotein associated with glycosphingolipid microdomains 1 | 1.915 |
| Iigp1: interferon inducible GTPase 1 | 1.896 |
| MPDZ: multiple PDZ domain protein | 1.893 |
| DNAH8: dynein, axonemal, heavy chain 8 | 1.886 |
| SLC4A4: solute carrier family 4, sodium bicarbonate cotransporter, member 4 | 1.86 |
| NRP2: neuropilin 2 | 1.783 |
| RNGTT: RNA guanylyltransferase and 5′-phosphatase | 1.782 |
| APCDD1: adenomatosis polyposis coli downregulated 1 | 1.756 |
| TMEM176B: transmembrane protein 176B | 1.745 |
| SHISA2: shisa homolog 2 (Xenopus laevis) | 1.706 |
| PLAGL1: pleomorphic adenoma gene-like 1 | 1.694 |
| CUX2: cut-like homeobox 2 | 1.689 |
| CALB1: calbindin 1, 28 kDa | 1.676 |
| CACHD1: cache domain containing 1 | 1.657 |
| SPC25: SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) | 1.653 |
| ENPP3: ectonucleotide pyrophosphatase/phosphodiesterase 3 | 1.65 |
| DTNA: dystrobrevin, α | 1.634 |
| HNMT: histamine N-methyltransferase | 1.628 |
| FGF12: fibroblast growth factor 12 | 1.625 |
| SCHIP1: schwannomin interacting protein 1 | 1.586 |
| NPPA: natriuretic peptide A | 1.576 |
| EPB41L4A: erythrocyte membrane protein band 4.1 like 4A | 1.565 |
| CST3: cystatin C | 1.546 |
| TRIM25: tripartite motif containing 25 | 1.544 |
| ZFHX3: zinc finger homeobox 3 | 1.541 |
| NPTX2: neuronal pentraxin II | 1.537 |
| SEMA4A: semaphorin 4A | 1.532 |
| SLC6A15: solute carrier family 6 (neutral amino acid transporter), member 15 | 1.529 |
| Genes downregulated in CGE-derived Prox1 loss-of-function cells in the E18.5 cortex | |
| Ddx3y: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked | 67.225 |
| EIF2S3: eukaryotic translation initiation factor 2, subunit 3 γ, 52 kDa | 51.606 |
| KDM5D: lysine (K)-specific demethylase 5D | 20.671 |
| VIP: vasoactive intestinal peptide | 14.152 |
| Uty: ubiquitously transcribed tetratricopeptide repeat gene, Y chromosome | 5.435 |
| TRIL: TLR4 interactor with leucine-rich repeats | 4.79 |
| CCK: cholecystokinin | 3.224 |
| RSPO2: R-spondin 2 | 2.076 |
| FGFR1: fibroblast growth factor receptor 1 | 1.908 |
| CRABP1: cellular retinoic acid binding protein 1 | 1.858 |
| TMEFF2: transmembrane protein with EGF-like and two follistatin-like domains 2 | 1.814 |
| CALB2: calbindin 2 | 1.787 |
| SPEG: SPEG complex locus | 1.776 |
| ID4: inhibitor of DNA binding 4, dominant negative helix-loop-helix protein | 1.762 |
| ROBO1: roundabout, axon guidance receptor, homolog 1 (Drosophila) | 1.746 |
| CRIM1: cysteine-rich transmembrane BMP regulator 1 (chordin-like) | 1.745 |
| MYO1B: myosin IB | 1.686 |
| NAV2: neuron navigator 2 | 1.671 |
| CPNE2: copine II | 1.671 |
| PTPRE: protein tyrosine phosphatase, receptor type, E | 1.665 |
| CACNA1G: calcium channel, voltage-dependent, T type, α-1G-subunit | 1.618 |
| GNAI1: guanine nucleotide binding protein (G-protein), α-inhibiting activity polypeptide 1 | 1.592 |
| CHST15: carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 | 1.585 |
| FAM134B: family with sequence similarity 134, member B | 1.579 |
| BCKDHB: branched chain keto acid dehydrogenase E1, β-polypeptide | 1.567 |
| Rcan2: regulator of calcineurin 2 | 1.555 |
| NRIP3: nuclear receptor interacting protein 3 | 1.545 |
| ZDHHC2: zinc finger, DHHC-type containing 2 | 1.542 |
| IL-18: interleukin 18 (interferon-γ-inducing factor) | 1.536 |
| CCRN4L: CCR4 carbon catabolite repression 4-like (S. cerevisiae) | 1.535 |
| WWC1: WW and C2 domain containing 1 | 1.528 |
| PRKCE: protein kinase C, ε | 1.519 |
| HSD11B1: hydroxysteroid (11-β) dehydrogenase 1 | 1.51 |
We FACS purified EGFP-labeled cells from the E18.5 cortices of control and Prox1 loss-of-function animals (Dlx6a-Cre; Prox1-C:EGFP/+ and /F) and subsequently performed a microarray expression analysis. Genes are shown upregulated and downregulated in cells lacking Prox1, respectively. Genes are shown for FDR-adjusted p -value (<0.05) and fold change (>1.5). Note that the values of gender-linked genes are indicated with boldface as male–female split happened by chance across control and Prox1 loss-of-function brains.