Figure 6.

Serum hormonal responses to a KP54 challenge in Gnaq^fl/fl and Gnaq^d/d mice. A–E, Serum FSH and LH levels, as well as testosterone levels in males only, were measured in 9-week-old virgin male and female Gnaq^d/d and Gnaq^fl/fl mice. Whereas intact males were used in this study, females were ovariectomized (OVX) 2 weeks before the KP54 challenge. Mice were administered either a single i.p. injection of 100 μl of vehicle (saline) or 100 μl of 100 nmol KP54/kg body weight. In a separate cohort of 9-week-old ovariectomized females and intact males, mice received two 100 μl, s.c. injections of the GnRH antagonist, Antide (1.25 μg/g body weight dissolved in sterile saline). Antide was administered at 24 and 1 h before i.p. injection of 100 μl of vehicle (saline) or 100 μl of 0.1 nmol KP54/g body weight. In all studies, 1 h after the KP54 injection, blood was collected by cardiac puncture. The number of mice used in these studies is indicated above each bar. An, Antide; S, saline. For A–E, a given KP54 response was compared with its respective saline control response or a given An + KP54 response was compared with its respective An + saline control response. Data were analyzed by Student's t test where p < 0.05 was considered statistically significant. *p < 0.05, ***p < 0.001, ****p < 0.0001. Error bars represent SEM. F, In another cohort of 12-week-old intact Gnaq^d/d males (n = 7), mice were challenged through central injections with KP54 (100 pmol) or the Gαq inhibitor, GP2A (5 nmol) and blood was collected 30 min after. For the GP-2A + KP54 group, mice were injected 30 min apart and blood was collected 30 min after the KP54 injection. For F, all treatments were compared with one another. Data were analyzed by two-way ANOVA followed by a Tukey post hoc test where different letters indicate significant differences between groups (p < 0.05). Error bars represent SEM.