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. 2015 Sep 16;35(37):12917–12931. doi: 10.1523/JNEUROSCI.2444-15.2015

Figure 4.

Figure 4.

ROSA-YFP reporter shows that DAT-Cre selectively expresses in dopaminergic neurons. a, Representative IHC images showing that YFP-ir in an animal with DAT-Cre-directed ROSA-YFP expression extensively colabels with TH-ir throughout the VTA, yet YFP expression was absent in sections from mice lacking Cre: green represents YFP-ir; red represents TH-ir; yellow represents double-label. b, Representative IHC images showing that YFP-ir in sections from an animal with DAT-Cre-directed YFP expression in the VTA does not colabel with GAD67-ir. Arrows point to GABAergic neurons. c, Representative IHC images showing that YFP-ir in an animal with DAT-Cre-directed YFP expression in the DRN colabels with TH-ir but not TPH-ir. Left panel, Imaged section of the DRN (modified from Paxinos and Franklin, 2008).