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. 2015 Mar 6;18(8):pyv010. doi: 10.1093/ijnp/pyv010

Figure 6.

Figure 6.

Blockade of the brain-derived neurotrophic factor (BDNF) signaling cascade by anti-BDNF infusion abolishes the antidepressant effects of tetramethylpyrazine (TMP). (A) Pre-infusion of an anti-BDNF antibody blocked the TMP-induced decrease of immobility in the forced swimming test in a dose-dependent manner between 5 and 20 µg/ml. (B) Pre-infusion of the anti-BDNF antibody (20 µg/ml) also prevented the TMP-induced decrease of immobility in the tail suspension test. (C) Chronic social defeat stress (CSDS)-treated mice were co-treated with TMP and anti-BDNF antibody (20 µg/ml) for 14 d. CSDS + TMP + anti-BDNF mice displayed significantly lower sucrose consumption than CSDS + TMP + Immunoglobulin Y (IgY) mice. (D) Co-treatment of TMP with the anti-BDNF antibody (20 µg/ml) blocked the antidepressant effect of TMP in the social interaction test. CSDS + TMP + anti-BDNF mice displayed significantly lower social interaction than CSDS + TMP + IgY mice. Results are expressed as means ± standard error of the mean (n = 10); * p < 0.05, ** p < 0.01; n.s., no significance. Comparison was made by one-way analysis of variance followed by a post hoc LSD test.