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. 2015 Sep 16;5:14081. doi: 10.1038/srep14081

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Assay parameters were determined using a plate coated with 18.5 × 10⁵ schizonts/mL and serum from malaria-naïve UK volunteers and Kenyan adults. (A) PMNs were isolated from whole blood and confirmed to be >75% pure by Giemsa stained slide. 100 μL of cell suspension was added to each well of the assay at 1 × 10⁷ PMNs/mL. (B) Scanning electron micrograph of PEMS preparation showing both merozoites (M) and schizonts (S). (C) ADRB activity induced by pooled serum from Kenyan (K_p) and UK adults (UK_p) was tested at different serum dilutions and is reported as maximum RLU. (D) ADRB activity induced by sera (5 Kenyan and 3 UK individual sera) diluted 1:50 before and after heat inactivation. (E) ADRB activity induced by a pool of sera from Kenyan adults and two individual Kenyan volunteers, and a pool of UK, malaria-naïve adults and two individual UK volunteers was assessed using plates coated with either PEMS (dark) or uninfected RBCs (light). Bars represent the mean of duplicate wells tested with the same PMN donor.