Table 2. Minimizing variation due to donor by indexing.
| AssayReadout | African (% R2donor) | African + UK (%R2donor) |
|---|---|---|
| RLU | 24.5 (17.8–33.8) | 12.5 (9.5–17.6) |
| Log10 RLU | 25.9 (18.7–34.9) | 19.4 (15.7–24.0) |
| RLUp | 3.5 (2.4–10.2) | 1.4 (1.2–4.6) |
| Log10 RLUp | 2.0 (1.7–7.4) | 5.3 (3.6–9.5) |
| RLUn | 44.3 (35.3–52.1) | 18.7 (13.8–24.9) |
| Log10 RLUn | 27.8 (21.6–35.6) | 6.8 (4.8–10.9) |
ADRB activity against PEMS was determined for 13 malaria-endemic African and 10 malaria-naïve UK serum or plasma samples using 8 different neutrophil donors. The Table reports percentage of assay output R2 explained by donor effects on African samples only (African), or both African and UK (African + UK) samples after no indexing (RLU); indexing against a positive hyper-immune Kenyan pool (RLUp); indexing against the mean of the 10 malaria-naïve UK samples (RLUn); and log transformation of data. Parentheses indicate 95% CIs.