Figure 2.

Integrin Signaling Is Sufficient to Rescue the BMP Phenotype in βPS⁻ Integrin Mutant Embryos

(A) Torso^DβPS_cyt construct, which can mimic integrin signaling in the absence of ligand binding.

(B and C) Overexpression of βPS and of Torso^DβPS_cyt in maternal/zygotic mys^XG43 mutant (βPS⁻) embryos can rescue expression of the peak threshold BMP target genes Race (B) and hnt (C). Rescue was quantified as the percentage increase in embryos with a wild-type expression pattern relative to a no transgene control (n = 3, > 50 embryos counted per genotype in each experiment; error bars are SEM). For details, see Supplemental Experimental Procedures. Asterisks denote significant difference from no transgene control (i.e., 0% rescue); ^∗∗p < 0.01, ^∗∗∗p < 0.001 (t test). Scale bars represent 50 μm.

See also Figure S3.