TABLE 1.
Catalytic properties of characterized PL2s and PL22s
SF, subfamily; pHΔ, experimentally determined pH optimum; ND, not determined; NA, not applicable.
| Enzyme | SF | pHΔ | Activity | Location | Metala | Reference |
|---|---|---|---|---|---|---|
| DdPL2B (PelW) | 2 | ND | Exo | Cytoplasm | Co2+, Mn2+, Ni2+ | Ref. 8, this studyb |
| PaePL2 | NA | 7.4 | Endo | Cytoplasm | Mg2+ | Ref. 7, this studyb |
| PaPL2B | 2 | ND | Exo | Cytoplasm | ND | This studyb |
| VvPL2 | 2 | 9.3 | Endo | Secreted | Mg2+ | This studyb |
| YePL2A | 1 | 9.6 | Endo | Secreted | Mg2+ | Ref. 3, this studyb |
| YePL2B | 2 | 8.6 | Exo | Cytoplasm | Mn2+ | Ref. 3, this studyb |
| DdPL22 (Ogl) | 1 | ND | Exo | Cytoplasm | Mn2+ | Ref. 8 |
| YePL22 | 1 | 7.6 | Exo | Cytoplasm | Mn2+ | Ref. 4 |
a The preferential metal that provides maximal activity in recovery assays.
b These assays were done with the exhaustive dialysis technique as opposed to the depletion-supplementation approach.