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. 2015 Jul 17;290(35):21244–21251. doi: 10.1074/jbc.R115.675165

FIGURE 4.

FIGURE 4.

An allosteric switch: the structure of the RNF146-UbcH5a complex. a, left, selected member of the solution ensemble of the RNF146 RING domain showing the E2 binding-incompetent state (PDB 2D8T). Right, crystal structure of the RNF146 RING-WWE domains bound to iso-ADPR (PDB 4QPL). Iso-ADPR binds between the RING and WWE domains. Binding of iso-ADPR results in the repositioning of Trp-66 and the extension of the helical region of the RING domain. b, UbcH5a binds to the RING domain of RNF146 when iso-ADPR binds between the RING and WWE domains. Displacement of Trp-66 allows for the interaction between RING and E2.