FIGURE 4.

CLEC18 are a secretory protein. A, detection of CLEC18 by flow cytometry and real-time PCR in human peripheral blood cells. Shadow, isotype control; dash line, anti-CLEC18 mAb. B and C, detection of CLEC18 by Western blot and real-time PCR in DC/Mφ (B) and cell lines (C). Arrow, CLEC18. D, the 293T cells were transfected with vector or pCMV-Tag4A-CLEC18A for 48 h, and the supernatant or lysates were incubated with anti-FLAG mAb to pulldown CLEC18, followed by Western blot analysis. E, detection of secretory CLEC18 in culture media and human sera. Culture supernatants (upper) of monocyte, dendritic cells, and macrophages and human sera (lower) were determined by ELISA (CUSABIO Life Science). ND, not determined.