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. 2015 Jul 9;290(35):21305–21319. doi: 10.1074/jbc.M115.649087

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Lys-122 and Lys-125 in the B. cenocepacia LPS signaling on murine and human TLR4·MD-2 complexes. An NF-κB-luciferase reporter assay stimulating HEK293 hTLR4·hMD-2, HEK293 mTLR4·mMD-2, and mTLR4·hMD-2 after 6 h with LPS_BC and LPS_BCΔAra was carried out. Stimulations for 6 h with E. coli LPS and with lipid IV_A were used as controls. The same protocol was used to stimulate HEK293 mTLR4·mMD-2 E1222K and HEK293 mTLR4·mMD-2 E122K/L125K cells. The data are pooled from two independent experiments done in triplicate. Bars indicate S.D.; statistical analysis was calculated for pairwise comparisons (*, p < 0.05; ***, p < 0.001). NS, no stimulation.