FIGURE 8.
TCAB1 transiently changes nuclear localization and association with hTR. A, HeLa ZZF-hTERT cells were synchronized at G1/S using double thymidine treatment and subsequently released into mitotic block using STLC. ZZF-TCAB1-hTR RNP levels were quantified by RT-qPCR, normalized to input hTR, and set relative to t = 0 (n = 3). B, HeLa F-TCAB1 cells were synchronized at G1/S and led into mitotic block as above. F-TCAB1-hTR RNP was assayed as described in A (n = 3). C, IF of coilin (green), TCAB1 (red), and DNA (black/blue) in HeLa F-TCAB1 cells under STLC induced mitotic block. D, IF of TCAB1 (green) and DNA (blue) in HeLa F-TCAB1 cells released from STLC mitotic block. The negative control sample (con) was the asynchronously dividing parental HeLa cells. n.s., not significant.