FIGURE 9.

The hTERT-hTR RNP does not undergo subunit exchange. A, HeLa ZZF-hTERT cells were synchronized at G₁/S using double thymidine block. Cells were released from block and after 1 h were transfected with empty vector (no competitor, mock) or competitor Myc-hTERT plasmid. Levels of hTR bound to ZZF-hTERT and the competing Myc-hTERT were assayed using RT-qPCR at 10 and 18 h post-release. hTR values were normalized to input hTR and set relative to the corresponding mock transfection sample (n = 3). QTRAP used total cell extract (n = 3). B, HeLa ZZF-hTERT cells were synchronized at prometaphase using STLC following a single round of thymidine block. One hour post-release, cells were transfected with empty vector or competitor Myc-hTERT plasmid. Bound hTR was assayed as above (n = 3). n.s., not significant.