FIGURE 4.

NAADP-evoked Ca²⁺ release from acidic stores modulates membrane potential. A and B, membrane potential (Em) recordings from β cells in small clusters exposed to 3 mm glucose. NAADP-AM (60 nm) was applied as indicated in B. C, Ned-19 (100 μm) abolishes the typical electrical activity evoked by 10 mm glucose in a single pancreatic β cell. D, typical electrical activity evoked by 10 mm glucose in a single β cell is reversibly abolished by bafilomycin (3 μm). Traces are representative of results obtained from three (A), six (B), and seven (C and D) single β cells. All traces represent different cells. *, p < 0.05, Student's t test.