FIGURE 4.

A. oris VKOR is required for type 1 fimbrial assembly. A–F and H, A. oris cells of various strains grown in the absence or presence of cystine were subject to IEM as described in the legend to Fig. 2 with α-FimP (A–C and H) or α-FimQ (D–F). Scale bars indicate 0.5 μm. G, the medium (S) and cell wall (W) fractions were collected from A. oris and its isogenic derivatives grown with (100 μg/ml) or without cystine. Protein samples were analyzed by immunoblotting with α-FimP.