FIGURE 10.
Analysis of collagen cross-linking in skin. Back skin from 3-month-old Fbln4+/+, Fbln4+/E57K, and Fbln4E57K/E57K mice (two of each genotype) was sequentially extracted in 1 m NaCl and 0.5 m acetic acid at a fixed ratio of 100 mg wet tissue/1 ml of extraction solution. Extracts were run on reduced 4–20% SDS-polyacrylamide gradient gels and stained with Coomassie Blue. A, 1.0 m NaCl extract. Each lane was loaded with 10 μl of extract from a separate mouse. B, 0.5 m acetic acid extract. Each lane was loaded with 6 μl of extract from a separate mouse. α1 and α2, un-cross-linked type I collagen chains; β11 and β12, divalently cross-linked collagen; γ, trivalently cross-linked collagen.