FIGURE 3.

Localization of various INCENP SAH mutants. Micrographs of cells from stable cell lines expressing GFP-TrAP-INCENP^{WT SAH} (A–C), GFP-TrAP-INCENP^{N-half SAH} (D–F), and GFP-TrAP-INCENP^{Double SAH} (G–I) are shown. INCENP is shown in green, DNA is shown in blue, and tubulin or CENP-T is shown in red. Yellow arrows point to the faint GFP signal of GFP-TrAP-INCENP^{WT SAH} around centrosomes. Scale bar, 5 μm. J, quantification of localization of various INCENP mutants. Twenty cells were counted for each sample. The average of two independent experiments is shown. K, quantification of histone H3 Ser¹⁰ phosphorylation status of various INCENP mutants in 12–20 cells each at prometaphase. p values were calculated based on Student's t test with unpaired variants. AU, absorbance units.