FIGURE 2.

The mucin-like domain of gC of HSV-1 influences the virus sensitivity to sulfated oligosaccharides. The HSV-1 mutant lacking the mucin-like domain in gC (KOS-gCΔmuc) and native KOS strain were tested for sensitivity to the sulfated oligosaccharides of heparin (a) and PI-88 (b) by the viral plaque-forming unit (PFU) reduction assay. c, the neuraminidase-treated viruses (KOS-N and KOS-gCΔmuc-N) as well as mock-treated viruses were tested for their sensitivity to heparin. For each assay, two separate experiments were performed, and the data are expressed as the percentage of the number of viral plaques found in the presence of the test compounds, relative to those developed in mock-treated infected cells. Error bars, S.D.