Binding of gC to surface-immobilized GAGs as measured with SPR.
a, real-time SPR response showing binding of native gC and gC lacking the mucin-like region (gCΔmuc) to surface-immobilized b-CS (dotted line), b-HA (dashed line), and b-sHA (solid line). The protein concentration was 11 nm, and onset of rinsing is marked with an arrow. b, mean and S.D. values for at least three measurements.