FIGURE 2.

Identification of interaction sites in the FtsQ_pB_pL_p complex by BAMG cross-linking. Purified FtsQ_pB_pL_p complexes were cross-linked in vitro using the bifunctional cross-linker BAMG. After cross-linking, the samples were treated with trypsin, and cross-linked peptides were purified and analyzed by LC-MS/MS. On the surface of FtsQ, BAMG cross-linked residues are depicted in dark blue (FtsB) and purple (FtsB/FtsL). Residues cross-linked by photo cross-linking (13) are depicted in light blue (FtsB), light red (FtsL), and pink (FtsB/FtsL). Model (Protein Data Bank code 2VH1) was created using PyMOL (49).