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. 2015 Jul 16;290(35):21724–21731. doi: 10.1074/jbc.M115.639732

FIGURE 4.

FIGURE 4.

The interaction with TNFR1 is necessary for TNF-α- and Tag7-Hsp70-induced cell death. A, the cytotoxic effects of TNF-α and Tag7-Hsp70 (1 nm each) on both apoptotic (3 h) and necroptotic (20 h) cell death were inhibited after the L-929 cells were preincubated with anti-TNFR1 antibodies (100 nm). B, the same experiment was performed on the 3-h-sensitive clones for the apoptotic (3 h) cell death and on the 20-h-sensitive clones for the necroptotic (20 h) cell death. C, preincubation of TNF-α (0.05 nm) and Tag7-Hsp70 (0.1 nm) with increasing concentrations of sTNFR1 inhibits their cytotoxic activity in L-929 cells. D, TNFR1 knockdown blocks the cytotoxic effect of TNF-α and Tag7-Hsp70 (see “Experimental Procedures”). All data are presented as the means ± S.E. from at least five independent assays. p values of less than 0.05 were considered significant (*, p < 0.05).