Peroxidasin, MPO, and EPO associate with extracellular matrix.
a–c, immunoblotting of isolated PFHR-9 matrix after overlay with transiently transfected HEK293T cells demonstrates the presence of MPO, EPO, and PXDN in the detergent and high salt-insoluble ECM fraction. d, actin immunoblotting reveals the presence of actin only in the deoxycholate soluble cell lysate, but not in the culture media or detergent-high salt insoluble matrix fraction. Blots are representative of 4 independent experiments.