TABLE 2.

Dextran-Csat value, autoxidation rate, and thermal stability of Hb S and recombinant mutants

	Dextran-Csat	Autoxidation rate	Thermal stabilitya
	g/dl	h−1
Hb A	NDb	0.00086 ± 0.0002	5.8 ± 0.1
Hb S	2.95 ± 0.10	0.0029 ± 0.0002	15.7 ± 0.2
rHb (βE6V/αH20R)	1.86 ± 0.20 2	0.0024 ± 0.0001	14.1 ± 0.2
rHb (βE6V/αH20Q)	3.04 ± 0.17	0.0023 ± 0.0002	13.9 ± 0.4
rHb (βE6V/αH50Q)	11.78 ± 0.59	0.0031 ± 0.0007	20.6 ± 1.0
rHb (βE6V/αH20R/αH50Q)	7.11 ± 0.66	0.0023 ± 0.0001	15.9 ± 0.9
rHb (βE6V/αH20Q/αH50Q)	10.89 ± 0.83	0.0025 ± 0.0002	12.0 ± 0.3

^a Percentage denatured proteins after heating at 60 °C for 10 min.

^b ND, not determined.